Aurora A anticorps

N° du produit ABIN6971419

Cet anticorps anti-Aurora A est un anticorps Souris Monoclonal détectant Aurora A dans WB, IF, ICC, IP et FACS. Adapté pour Humain.

Aperçu rapide pour Aurora A anticorps (ABIN6971419)

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Aurora A est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FACS)

Clone: 35C1

Détail du produit anti-Aurora A anticorps

Attributs du produit: Aurora A (Aurora Kinase A) is a mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis. Aurora A antibody (mAb) (Clone 35C1) was raised in a Mouse host. It has been validated for use in Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and Western blot, it has been shown to react with Human samples.

Purification: Protein A Chromatography

Immunogène: This antibody was raised against full-length recombinant human Aurora A protein.

Isotype: IgG2b

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Stockage

Buffer: Purified IgG in PBS with 30 % glycerol and 0.035 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Détails sur Aurora A

Antigène: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Autre désignation: Aurora A

Poids moléculaire: 50 kDa

NCBI Accession: NP_003591

Pathways: Cycle Cellulaire, Asymmetric Protein Localization